How to Reconstitute Peptides
A complete guide to mixing BPC-157, TB-500, and other peptides with bacteriostatic water — including exact amounts, dose calculations, and storage.
Before You Begin
Only use bacteriostatic water — not tap water, saline, or regular sterile water for multi-dose vials. Work in a clean environment. Never reuse syringes.
Gather Your Supplies
You need: lyophilized peptide vial, bacteriostatic water vial, alcohol swabs, and insulin syringes (29–31 gauge, 1mL). Everything must be sterile. Never reuse syringes.
Tip: Bacteriostatic water (BAC water) is required — not regular sterile water, not saline. BAC water stays sterile for 28 days after opening.
Wipe All Vial Tops
Swab the rubber stopper on both the peptide vial and the bacteriostatic water vial with a fresh alcohol swab. Allow to air dry for 30 seconds. This prevents contamination.
Tip: Use a new swab for each vial. Do not touch the rubber stopper with your fingers after swabbing.
Draw the Bacteriostatic Water
Draw the amount of BAC water you need into a fresh insulin syringe. For a standard 5mg peptide vial, draw 1mL (100 units on a 1mL syringe). See the dosage table below for other amounts.
Tip: More water = lower concentration per unit. Less water = higher concentration. Standard is 1mL per 5mg vial.
Inject Water Into the Peptide Vial
Insert the syringe through the rubber stopper at an angle. Slowly inject the water down the inside wall of the vial — never directly onto the peptide powder. The powder will dissolve gradually.
Tip: Never spray water directly onto the powder — this can damage the peptide structure. Let it run down the glass wall gently.
Dissolve — Do Not Shake
Gently swirl the vial in a circular motion until the powder fully dissolves into a clear solution. This typically takes 30–60 seconds. The solution should be clear and colorless.
Tip: Never shake the vial vigorously — this can denature the peptide. Gentle swirling or rolling between your palms is correct.
Label and Store
Write the reconstitution date on the vial. Store in the refrigerator at 2–8°C. Use within 30 days. For longer storage, freeze at -20°C — the peptide will last 6+ months frozen.
Tip: Keep away from direct light. Do not leave reconstituted peptides at room temperature for extended periods.
Peptide Dose Reference Table
How much to draw from a 1mL insulin syringe for 250mcg or 500mcg doses at different concentrations.
| BAC Water | Vial Size | Concentration | 250mcg dose | 500mcg dose |
|---|---|---|---|---|
| 0.5mL | 2mg | 4,000mcg/mL | 6.25μL (0.6 IU) | 12.5μL (1.25 IU) |
| 1mL | 2mg | 2,000mcg/mL | 12.5μL (1.25 IU) | 25μL (2.5 IU) |
| 1mL | 5mg | 5,000mcg/mL | 50μL (5 IU) | 100μL (10 IU) |
| 2mL | 5mg | 2,500mcg/mL | 100μL (10 IU) | 200μL (20 IU) |
| 1mL | 10mg | 10,000mcg/mL | 25μL (2.5 IU) | 50μL (5 IU) |
| 2mL | 10mg | 5,000mcg/mL | 50μL (5 IU) | 100μL (10 IU) |
IU = units on a 1mL insulin syringe (100 IU = 1mL). μL = microliters.
Reconstitution FAQ
How much bacteriostatic water do I add to BPC-157?+
The most common approach: add 1mL of bacteriostatic water to a 5mg BPC-157 vial. This creates a concentration of 5,000mcg/mL. For a 250mcg dose, draw 50μL (5 IU on a 1mL insulin syringe). For a 500mcg dose, draw 100μL (10 IU). You can use more water for a lower concentration if you prefer smaller volumes.
Can I use regular sterile water instead of bacteriostatic water?+
Technically you can use regular sterile water, but only if you use the entire vial in a single session — regular sterile water has no preservative and will allow bacterial growth once opened. Bacteriostatic water (0.9% benzyl alcohol) maintains sterility for 28 days and is the correct choice for any multi-dose peptide vial.
How long does reconstituted BPC-157 last in the fridge?+
Reconstituted BPC-157 stored in bacteriostatic water lasts 30 days at refrigerator temperature (2–8°C). For storage beyond 30 days, freeze the vial at -20°C where it will last 6+ months. Avoid repeated freeze-thaw cycles — if you need long-term storage, aliquot into smaller vials before freezing.
What does IU mean on an insulin syringe?+
IU (International Units) is the measurement scale on insulin syringes. On a standard 1mL insulin syringe, 100 IU = 1mL = 1,000μL. So 1 IU = 10μL. If you reconstitute 5mg in 1mL (5,000mcg/mL), drawing to the "5 IU" mark gives you 50μL = 250mcg of peptide.
How do I know if my peptide dissolved correctly?+
Correctly reconstituted peptide will be a clear, colorless (or very slightly yellow-tinted) liquid with no visible particles. Cloudiness or particles may indicate contamination, incorrect water type, or a damaged peptide. If the solution looks cloudy or discolored, do not use it.
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